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br Results and discussion A
2020-08-19
Results and discussion A systematic search for genes encoding H+-PPases was carried out by PCR using genomic DNA from a wide range of protozoa as templates and degenerate oligonucleotides, designed from amino LDN 57444 domains conserved in H+-PPases of higher plants and the proteobacterium R. ru
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Besides the regulation of the stress responses CRF and the
2020-08-19
Besides the regulation of the stress responses, CRF and the urocortins have been implicated in drug addiction (Bruijnzeel and Gold, 2005, Sarnyai et al., 2001). For instance nicotine, the addictive substance of tobacco, can activate the HPA axis, just like any other stressor may do, although its imp
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This approach is similar to that suggested
2020-08-19
This approach is similar to that suggested by the previous edition of the GL [6]. Not surprisingly, the questions to be answered are the same (i.e., was the event TLOC? In cases of TLOC, is this Phosphatase Inhibitor Cocktail 3 (100X in DMSO) of syncopal or non-syncopal origin? In cases of suspecte
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Key molecules in the EP second messenger signaling cascade t
2020-08-19
Key molecules in the EP1 second messenger signaling cascade that are necessary for potentiation of GluK2/GluK5 were identified. We also identified three GluK5 serines located in a membrane-proximal C-terminal domain that, when mutated to the phosphomimetic aspartate and expressed with GluK2, rendere
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If the effects described here are present
2020-08-19
If the effects described here are present in catalysis, their empirical confirmation poses many challenges. Cat state correlations are fragile outside an environment especially suited for their preservation. As present day observational techniques whether in vivo or in vitro were not designed bearin
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When six chemical inhibitors were incubated with TRB or
2020-08-19
When six chemical inhibitors were incubated with TRB or TRC and human liver microsomal preparation, HLM2, the results showed that ketoconazole (a CYP3A4 inhibitor) was very effective in inhibiting 4β-C hydroxylation of TRB and TR, whereas sulfaphenazole (a CYP2C9 inhibitor) and quinidine (a CYP2D6 i
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Protein expression and purification A mL overnight
2020-08-18
Protein expression and purification. A 3 mL overnight culture of E. coli BL21 (DE3) pLysS pGEX-6P-3-A1S_0222 was grown in Luria-Bertani (LB)-Medium (10 g/L tryptone, 5 g/L yeast extract, 5 g/L NaCl, pH 7.4, 100 μg/mL Amp) at 37 °C and 160 rpm. The overnight culture was diluted 1:100 into 200 mL LB (
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pituitary adenylate cyclase-activating peptide br Compliance
2020-08-18
Compliance with ethical standards This work was conducted in compliance with ethical standards. Conflict of interest Funding The work was funded by extra mural research grant (EMR/2016/005135) from Science and Engineering Research Board, Government of India. Acknowledgements Introduc
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br Materials and methods br Results br Discussion Cysteine p
2020-08-18
Materials and methods Results Discussion Cysteine proteases are upregulated under oxidative stress and play an important role in the preservation of cellular metabolism (Usui et al., 2007). Cysteine proteases are also important under abiotic stresses for plants to degrade proteins denatured
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Because flavonoids are widely considered to contribute to
2020-08-18
Because flavonoids are widely considered to contribute to health benefits in humans, including anti-inflammatory, antibacterial, antiviral, anticancer, antiplatelet properties, and free radical scavenging capacity (Xiao et al., 2011), efforts have frequently been made to increase their bioavailabili
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br Acknowledgement AM and SK thank ICMR New Delhi
2020-08-18
Acknowledgement AM and SK thank ICMR, New Delhi, India for the financial assistance BIC/12(32)/2012. SK is the recipient of JRF from the project. RK and GJ thank Bristol-Myer Squibb (Grant # 34003085), USA for financial support. Introduction Proliferating Cell Nuclear Antigen (PCNA) is a high
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Recoverin is a kDa retinal protein that binds to
2020-08-18
Recoverin is a 23-kDa retinal protein that binds to calcium. It regulates the C527 mg of rhodopsin during visual transduction within photoreceptor cells. Since the pathogenicity of anti-recoverin antibody is potent, anti-recoverin CAR patients often have progressive visual impairments, characterize
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WWL 70 The identification of CCR B cells within the LZ as
2020-08-18
The identification of CCR6+ WWL 70 within the LZ as direct progenitors of MBCs provides a new window into the evolution of immune responses. In practical terms, flow-cytometric detection of CCR6+ GC B cells could provide a means of rapidly assessing vaccine efficacy when applied to fine needle aspir
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These studies established IAP proteins as dimeric
2020-08-17
These studies established IAP proteins as dimeric RING E3 ligases, but did not account for the essential role of dimerization. In IAPs and related E3s, such as RNF4 and MDM2, dimerization not only depends on contacts from the core RING domain but also residues N- and C-terminal to the RING domain (B
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br Discussion The aim of this study was to establish
2020-08-17
Discussion The aim of this study was to establish staining protocols to enhance the visualization of a dental filling or its remaining in forensic dentistry. Teeth and dental materials resist to most of environmental and disaster conditions, therefore the forensic odontology exam is essential in
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